A lay summary of Monika's PhD research will be available shortly!
Highly repetitive DNA is prone to form unstable secondary structures within itself, and is recalcitrant to many of the standard techniques in molecular genetics. As a result, research in this field relies upon tools that are labour-intensive and technically challenging - but which produce beautiful results!
One such example, is the need to purify DNA with long expanded repeat tracts. Cesium chloride DNA purification provides one of the best methods for purifying large amounts of DNA (whereas column-based purification methods tends to shear the DNA, or bind the repetitive DNA significantly decreasing the yield). The video below shows one of the steps in the 4-to-5 day cesium chloride DNA purification protocol.
One such example, is the need to purify DNA with long expanded repeat tracts. Cesium chloride DNA purification provides one of the best methods for purifying large amounts of DNA (whereas column-based purification methods tends to shear the DNA, or bind the repetitive DNA significantly decreasing the yield). The video below shows one of the steps in the 4-to-5 day cesium chloride DNA purification protocol.